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Last updated: 2017/11/19
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ABCB5 polyclonal antibody

  • Catalog # : PAB9925
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ABCB5.
  • Immunogen:
  • A synthetic peptide corresponding to internal region of human ABCB5.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:80000-1:85000)
    Western Blot (1:10000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using ABCB5 polyclonal antibody (Cat # PAB9925) shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate.
    No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown).
    A 3-8% Tris-acetate gel was used for separation.
    The arrowhead corresponds to 117 kDa ABCB5.
    The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.
    Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • AAW31630;Q515Q7
  • Gene Name:
  • ABCB5
  • Gene Alias:
  • ABCB5alpha,ABCB5beta,EST422562
  • Gene Description:
  • ATP-binding cassette, sub-family B (MDR/TAP), member 5
  • Gene Summary:
  • ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM
  • Other Designations:
  • ATP-binding cassette protein,ATP-binding cassette, sub-family B, member 5,P-glycoprotein ABCB5
  • Gene Pathway
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