ATR polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot using ATR polyclonal antibody (Cat # PAB9924) in HeLa cell nuclear extract (Lane 1).
Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation).
A 4-8% gradient gel was used for separation.
Goat serum was used at 5% for blocking.
The arrowhead corresponds to 301 kDa ATR.
The primary antibody was used at a 1 : 1,400 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATR.
Immunogen
A synthetic peptide corresponding to amino acids 262-277 of human ATR.
Host
Rabbit
Reactivity
Clawed frog, Dog, Human, Monkey, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:70000)
Western Blot(1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot using ATR polyclonal antibody (Cat # PAB9924) in HeLa cell nuclear extract (Lane 1).
Lane 2 shows negligible staining after pre-incubation of antibody with the immunizing peptide (50 ug peptide for 1 h at room temperature followed by centrifugation).
A 4-8% gradient gel was used for separation.
Goat serum was used at 5% for blocking.
The arrowhead corresponds to 301 kDa ATR.
The primary antibody was used at a 1 : 1,400 dilution.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATR
Entrez GeneID
545Gene Name
ATR
Gene Alias
FRP1, MEC1, SCKL, SCKL1
Gene Description
ataxia telangiectasia and Rad3 related
Gene Ontology
HyperlinkOther Designations
FRAP-related protein-1|MEC1, mitosis entry checkpoint 1, homolog|Rad3 related protein|ataxia telangiectasia and Rad3 related protein|protein kinase ATR
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Interactome
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Pathway
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Disease
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Publication Reference
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Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint.
Bruno T, De Nicola F, Iezzi S, Lecis D, D'Angelo C, Di Padova M, Corbi N, Dimiziani L, Zannini L, Jekimovs C, Scarsella M, Porrello A, Chersi A, Crescenzi M, Leonetti C, Khanna KK, Soddu S, Floridi A, Passananti C, Delia D, Fanciulli M.
Cancer Cell 2006 Dec; 10(6):473.
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ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling.
Stiff T, Walker SA, Cerosaletti K, Goodarzi AA, Petermann E, Concannon P, O'Driscoll M, Jeggo PA.
The EMBO Journal 2006 Dec; 25(24):5775.
Application：KA, IP, WB, Human, Seckel whole‐cell extracts.
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RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint.
Olson E, Nievera CJ, Klimovich V, Fanning E, Wu X.
The Journal of Biological Chemistry 2006 Dec; 281(51):39517.
Application：WB, Human, U-2 OS cells.
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Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint.
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