Product Browser

Last updated: 2014/7/14
  • Related Product Showcase
  • By Research Area

Product Compare

Product Compare Cancel   Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

PTENP1 polyclonal antibody

  • Catalog # : PAB9923
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against partial recombinant PTENP1.
  • Immunogen:
  • Recombinant protein corresponding to N-terminus of human PTENP1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human, Macaque, Mouse, Opossum, Pufferfishes, Rat, Zebra fish
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:160000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using PTENP1 polyclonal antibody (Cat # PAB9923) shows detection of endogenous PTENP1 in whole cell lysates from human derived cell lines HeLa (Lane 4), HEK293 (Lane 5) and MCF-7 (Lane 6).
    The band at ~55 KDa (arrowhead) corresponds to PTENP1.
    Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.
    The identity of lower molecular weight bands in lane 4 is unknown.
    Briefly, each lane contains approximately 35 ug of lysate.
    Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System d
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • O43460;NP_000305;AAH38293
  • Gene Name:
  • PTENP1
  • Gene Alias:
  • PTEN-rs,PTEN2,PTH2,psiPTEN
  • Gene Description:
  • phosphatase and tensin homolog pseudogene 1
  • Other Designations:
  • -
  • RSS
  • YouTube
  • Linkedin
  • Facebook