PTENP1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using PTENP1 polyclonal antibody (Cat # PAB9923) shows detection of endogenous PTENP1 in whole cell lysates from human derived cell lines HeLa (Lane 4), HEK293 (Lane 5) and MCF-7 (Lane 6).
The band at ~55 KDa (arrowhead) corresponds to PTENP1.
Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.
The identity of lower molecular weight bands in lane 4 is unknown.
Briefly, each lane contains approximately 35 ug of lysate.
Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant PTENP1.
Immunogen
Recombinant protein corresponding to N-terminus of human PTENP1.
Host
Rabbit
Reactivity
Chimpanzee, Dog, Human, Macaque, Mouse, Opossum, Pufferfishes, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:160000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using PTENP1 polyclonal antibody (Cat # PAB9923) shows detection of endogenous PTENP1 in whole cell lysates from human derived cell lines HeLa (Lane 4), HEK293 (Lane 5) and MCF-7 (Lane 6).
The band at ~55 KDa (arrowhead) corresponds to PTENP1.
Lanes 1-3 were show the results of staining after the antibody was first pre-incubated with the immunizing peptide.
The identity of lower molecular weight bands in lane 4 is unknown.
Briefly, each lane contains approximately 35 ug of lysate.
Primary antibody was used at a 1 : 500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PTENP1
Entrez GeneID
11191Protein Accession#
O43460;NP_000305;AAH38293Gene Name
PTENP1
Gene Alias
PTEN-rs, PTEN2, PTH2, psiPTEN
Gene Description
phosphatase and tensin homolog pseudogene 1
Gene Ontology
HyperlinkOther Designations
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Publication Reference
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Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression.
Baak JP, Van Diermen B, Steinbakk A, Janssen E, Skaland I, Mutter GL, Fiane B, Lovslett K.
Human Pathology 2005 May; 36(5):555.
Application：IHC-P, Human, Human endometrioid endometrial adenocarcinoma.
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Epidermal growth factor and hypoxia-induced expression of CXC chemokine receptor 4 on non-small cell lung cancer cells is regulated by the phosphatidylinositol 3-kinase/PTEN/AKT/mammalian target of rapamycin signaling pathway and activation of hypoxia inducible factor-1alpha.
Phillips RJ, Mestas J, Gharaee-Kermani M, Burdick MD, Sica A, Belperio JA, Keane MP, Strieter RM.
The Journal of Biological Chemistry 2005 Jun; 280(23):22473.
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Alterations of PTEN/MMAC1, a candidate tumor suppressor gene, and its homologue, PTH2, in small cell lung cancer cell lines.
Kim SK, Su LK, Oh Y, Kemp BL, Hong WK, Mao L.
Oncogene 1998 Jan; 16(1):89.
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Lack of PTEN expression in endometrial intraepithelial neoplasia is correlated with cancer progression.
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