Antibody Reactive Against Native Purified Protein.
ELISA (1:10000) Western Blot (0.5-1.0 ug/mL) Immunocytochemistry (1:50-1:200) Immunohistochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunoprecipitation (5ug/300uL) The optimal working dilution should be determined by the end user.
In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blotting using Beta-Galactosidase polyclonal antibody (Cat # PAB9921) shows a band at ~117 kDa (Lanes 1 - 3) corresponding to 60 ng, 30 ng and 15 ng, respectively of Beta-Galactosidase present in partially purified preparations (arrowhead). Lane 4 shows no cross reactivity with proteins present in a non-specific control E.coli lysate.