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Last updated: 2016/10/23
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His2Av (phospho S137) polyclonal antibody

  • Catalog # : PAB9920
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phsphopeptide of His2Av.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S137 of drosophila His2Av.
  • Host:
  • Rabbit
  • Reactivity:
  • Fruit fly
  • Specificity:
  • Reactivity occurs against Drosophila H2AvD pS137 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:16000)
    Western Blot (1:400-1:1600)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head).
    Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1 : 500.
    Washes and reaction with secondary antibody followed incubation.
    Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
    Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Protein Accession#:
  • S08118;P08985;NP_524519
  • Gene Name:
  • His2Av
  • Gene Alias:
  • CG5499,H2A.X,H2A.Z,H2AV,H2AX,H2AZ,H2AvD,His2AvD,l(3)05146,l(3)810,l(3)97Dd,l(3)L1602
  • Gene Description:
  • Histone H2A variant
  • Other Designations:
  • H2A variant,Histone H2A variant CG5499-PA,histone
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