His2Av (phospho S137) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head).
Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 500.
Washes and reaction with secondary antibody followed incubation.
Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phsphopeptide of His2Av.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S137 of drosophila His2Av.
Host
Rabbit
Reactivity
Fruit fly
Specificity
Reactivity occurs against Drosophila H2AvD pS137 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:400-1:1600)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head).
Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1 : 500.
Washes and reaction with secondary antibody followed incubation.
Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection.
Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.Enzyme-linked Immunoabsorbent Assay
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Gene Info — His2Av
Entrez GeneID
43229Protein Accession#
S08118;P08985;NP_524519Gene Name
His2Av
Gene Alias
CG5499, H2A.X, H2A.Z, H2AV, H2AX, H2AZ, H2AvD, His2AvD, l(3)05146, l(3)810, l(3)97Dd, l(3)L1602
Gene Description
Histone H2A variant
Gene Ontology
HyperlinkOther Designations
H2A variant|Histone H2A variant CG5499-PA|histone
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Publication Reference
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A deficiency screen for dominant suppressors of telomeric silencing in Drosophila.
Mason JM, Ransom J, Konev AY.
Genetics 2004 Nov; 168(3):1353.
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Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster.
Kimura K, Kodama A, Hayasaka Y, Ohta T.
Development 2004 Apr; 131(7):1597.
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DNA double-strand break-induced phosphorylation of Drosophila histone variant H2Av helps prevent radiation-induced apoptosis.
Madigan JP, Chotkowski HL, Glaser RL.
Nucleic Acids Research 2002 Sep; 30(17):3698.
Application：WB-Ce, Drosophila, S2 cells.
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A deficiency screen for dominant suppressors of telomeric silencing in Drosophila.
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