Rabbit polyclonal antibody raised against synthetic phsphopeptide of His2Av.
Synthetic phosphopeptide corresponding to residues surrounding S137 of drosophila His2Av.
Reactivity occurs against Drosophila H2AvD pS137 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated Drosophila H2AvD is minimal by ELISA.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:4000-1:16000) Western Blot (1:400-1:1600) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using His2Av (phospho S137) polyclonal antibody (Cat # PAB9920) shows detection of aband at ~ 15 KDa corresponding to Phospho-His2Av S137 (Lane 2 arrow-head). Lanes contain either mock-irradiated (Lane 1) or 4000-RAD gamma irradiated (Lane 2) Drosophila melanogaster (3rd instar) larvae brainWC lysate separated on by SDS-PAGE and transferred to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1 : 500. Washes and reaction with secondary antibody followed incubation. Use HRP conjugated Gt-a-Rabbit IgG [H&L] MX and ECL for detection. Personal Communication. Yikang Rong, NIH, CCR, Bethesda, MD.