Bub1b polyclonal antibody
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More Files
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Immunoprecipitation
Immunoprecipitation blot analysis of Bub1b expression on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry with Bub1b polyclonal antibody.
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Specification
Product Description
Sheep polyclonal antibody raised against partial recombinant Bub1b.
Immunogen
Recombinant protein corresponding to amino acids 232-603 of mouse Bub1b.
Host
Sheep
Reactivity
Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunoprecipitation
Immunoprecipitation blot analysis of Bub1b expression on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flow cytometry with Bub1b polyclonal antibody. -
Gene Info — Bub1b
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Publication Reference
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Mutation and expression analysis of human BUB1 and BUB1B in aneuploid breast cancer cell lines.
Myrie KA, Percy MJ, Azim JN, Neeley CK, Petty EM.
Cancer Letters 2000 May; 152(2):193.
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The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner.
Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R.
Genomics 1999 Jan; 55(1):113.
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Mutation and expression analysis of human BUB1 and BUB1B in aneuploid breast cancer cell lines.
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