Grin2c polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k Grin2c subunit of the NMDA receptor. Using Grin2c polyclonal antibody (Cat # PAB9705).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Grin2c.
Immunogen
Recombinant protein corresponding to N-terminus rat Grin2c.
Host
Rabbit
Theoretical MW (kDa)
140
Reactivity
Human, Mouse, Rat
Specificity
Also labels the ~180k NR2A and the ~180k NR2B subunits of the NMDA receptor.
Form
Lyophilized
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000-2000)
Immunofluorescence (1:1000-2000)
Immunoprecipitation (3 ul/200 ug lysate)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 5 mM ammonium bicarbonate
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 50 uL of PBS, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of 10 ug of rat cerebellar lysate showing specific immunolabeling of the ~140k Grin2c subunit of the NMDA receptor. Using Grin2c polyclonal antibody (Cat # PAB9705).Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunoprecipitation
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Gene Info — Grin2c
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Publication Reference
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VEGFD Protects Retinal Ganglion Cells and, consequently, Capillaries against Excitotoxic Injury.
Schlüter A, Aksan B, Diem R, Fairless R, Mauceri D.
Molecular Therapy. Methods & Clinical Development 2019 Dec; 17:281.
Application：WB-Ce, Mouse, bEnd.3 cells, Hippocampal neurons.
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Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function.
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD.
The Journal of Biological Chemistry 2003 Jan; 278(13):11020.
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NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
Carroll RC, Zukin RS.
Trends in Neurosciences 2002 Nov; 25(11):571.
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Trafficking of NMDA receptors.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.
Annual Review of Pharmacology and Toxicology 2002 Jan; 43:335.
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VEGFD Protects Retinal Ganglion Cells and, consequently, Capillaries against Excitotoxic Injury.
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