Grin2a polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k Grin2a subunit of the NMDA receptor. Using Grin2a polyclonal antibody (Cat # PAB9699).
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant Grin2a.
Immunogen
Recombinant protein corresponding to C-terminus rat Grin2a.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Human, Mouse, Rat
Specificity
Recognizes human, mouse and rat forms of the NR2B subunits of NMDAR.
Form
Lyophilized
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000-2000)
Immunoprecipitation (3 ul/200 ug lysate)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 5 mM ammonium bicarbonate
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with PBS, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k Grin2a subunit of the NMDA receptor. Using Grin2a polyclonal antibody (Cat # PAB9699).Immunohistochemistry (Frozen sections)
Immunoprecipitation
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Gene Info — Grin2a
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Publication Reference
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Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function.
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD.
The Journal of Biological Chemistry 2003 Jan; 278(13):11020.
Application：IP, WB, Rat, Rat hippocampus.
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NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
Carroll RC, Zukin RS.
Trends in Neurosciences 2002 Nov; 25(11):571.
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Trafficking of NMDA receptors.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.
Annual Review of Pharmacology and Toxicology 2002 Jan; 43:335.
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Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function.
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