Kcnc1 (phospho S503) polyclonal antibody
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More Files
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Immunofluorescence
Immunostaining of medial nucleus of the trapezoid body (MNTB) cells with Kcnc1 (phospho S503) polyclonal antibody (Cat # PAB9689). The left panel shows control cells. The right panel shows cells that have been exposed to the Kcnc1 activator PMA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Kcnc1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S503 of mouse and rat Kcnc1.
Host
Rabbit
Theoretical MW (kDa)
100
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunostaining of medial nucleus of the trapezoid body (MNTB) cells with Kcnc1 (phospho S503) polyclonal antibody (Cat # PAB9689). The left panel shows control cells. The right panel shows cells that have been exposed to the Kcnc1 activator PMA. -
Publication Reference
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Loss of Kv3.1 tonotopicity and alterations in cAMP response element-binding protein signaling in central auditory neurons of hearing impaired mice.
von Hehn CA, Bhattacharjee A, Kaczmarek LK.
Journal of Neuroscience 2004 Feb; 24(8):1936.
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Cell type-specific expression of the Kv3.1 gene is mediated by a negative element in the 5' untranslated region of the Kv3.1 promoter.
Gan L, Hahn SJ, Kaczmarek LK.
Journal of Neurochemistry 1999 Oct; 73(4):1350.
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Voltage-gated potassium channels: from hyperexcitability to excitement.
Pongs O.
FEBS Letters 1999 Jun; 452(1-2):31.
Application：WB-Ce, WB-Tr, Human, Mammalian cells.
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Loss of Kv3.1 tonotopicity and alterations in cAMP response element-binding protein signaling in central auditory neurons of hearing impaired mice.
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