Gabrb3 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot (tissue lysate) analysis of rat brain lysate showing specific immunolabeling of the β3-subunit of the GABAA-R.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunostaining of mouse retina showing specific labeling of the GABAA β3 subunit (green), calbindin (red) and DNA (blue).
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Specification
Product Description
Rabbit polyclonal antibody raised against a recombinant Gabrb3.
Immunogen
Recombinant protein corresponding to rat Gabrb3.
Host
Rabbit
Theoretical MW (kDa)
53
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot (tissue lysate) analysis of rat brain lysate showing specific immunolabeling of the β3-subunit of the GABAA-R.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunostaining of mouse retina showing specific labeling of the GABAA β3 subunit (green), calbindin (red) and DNA (blue). -
Gene Info — Gabrb3
Entrez GeneID
24922Protein Accession#
P63079Gene Name
Gabrb3
Gene Alias
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Gene Description
gamma-aminobutyric acid (GABA) A receptor, beta 3
Gene Ontology
HyperlinkGene Summary
O
Other Designations
Gamma-aminobutyric acid receptor beta 3|gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3|gamma-aminobutyric acid receptor, subunit beta 3|testis gamma-aminobutyric acid receptor subunit beta 3
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Publication Reference
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Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia.
Zhiqian Yu, Mai Sakai, Hotaka Fukushima, Chiaki Ono, Yoshie Kikuchi, Ryuta Koyama, Ko Matsui, Tomoyuki Furuyashiki, Satoshi Kida, Hiroaki Tomita.
Brain Research Bulletin 2022 Oct; 189:57.
Application：IF, Mouse, Mouse hippocampus.
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Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABA(A) receptor gamma2 subunit.
Ogris W, Poltl A, Hauer B, Ernst M, Oberto A, Wulff P, Hoger H, Wisden W, Sieghart W.
Biochemical Pharmacology 2004 Oct; 68(8):1621.
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Subunit composition and quantitative importance of GABA(A) receptor subtypes in the cerebellum of mouse and rat.
Poltl A, Hauer B, Fuchs K, Tretter V, Sieghart W.
Journal of Neurochemistry 2003 Dec; 87(6):1444.
Application：IP, WB, Human, Mouse, Rat, HEK 293 cells, Mouse and Rat cerebellar extracts.
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Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABA(A) receptor alpha1 subtype.
McKernan RM, Rosahl TW, Reynolds DS, Sur C, Wafford KA, Atack JR, Farrar S, Myers J, Cook G, Ferris P, Garrett L, Bristow L, Marshall G, Macaulay A, Brown N, Howell O, Moore KW, Carling RW, Street LJ, Castro JL, Ragan CI, Dawson GR, Whiting PJ.
Nature Neuroscience 2000 Jun; 3(6):587.
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Contextual fear conditioning regulates synapse-related gene transcription in mouse microglia.
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