Pak2 (phospho T402) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k Pak2 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Pak2 (phospho T402) polyclonal antibody (Cat # PAB9676). The immunolabeling of Pak2 is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Pak2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding phospho-T402 of rat Pak2.
Host
Rabbit
Theoretical MW (kDa)
~68 to ~70
Reactivity
Bovine, Dog, Human, Mouse, Rat
Specificity
Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with lambda phosphatase.
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 100 ug BSA per mL).
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
The peptide sequence used for immunogen is identical in Pak1, 2 and 3. Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.
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Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~68k to ~70k Pak2 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Pak2 (phospho T402) polyclonal antibody (Cat # PAB9676). The immunolabeling of Pak2 is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Pak2
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Publication Reference
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The mental retardation protein PAK3 contributes to synapse formation and plasticity in hippocampus.
Boda B, Alberi S, Nikonenko I, Node-Langlois R, Jourdain P, Moosmayer M, Parisi-Jourdain L, Muller D.
Journal of Neuroscience 2004 Dec; 24(48):10816.
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Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation.
Jakobi R, McCarthy CC, Koeppel MA, Stringer DK.
The Journal of Biological Chemistry 2003 Oct; 278(40):38675.
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Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation.
Jakobi R, Huang Z, Walter BN, Tuazon PT, Traugh JA.
European Journal of Biochemistry 2000 Jul; 267(14):4414.
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The mental retardation protein PAK3 contributes to synapse formation and plasticity in hippocampus.
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