Stxbp1 (phospho S515) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Stxbp1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S515 of rat Stxbp1.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~65k Stxbp1 protein phosphorylated at Ser515. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen, but not by the corresponding dephosphopeptide (not shown).Immunofluorescence
Stxbp1 (phospho S515) polyclonal antibody (Cat # PAB9675) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining of 14 DIV Cortical Neurons. -
Gene Info — Stxbp1
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Publication Reference
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Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.
Ciufo LF, Barclay JW, Burgoyne RD, Morgan A.
Molecular Biology of the Cell 2005 Feb; 16(2):470.
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Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo.
Liu J, Ernst SA, Gladycheva SE, Lee YY, Lentz SI, Ho CS, Li Q, Stuenkel EL.
The Journal of Biological Chemistry 2004 Dec; 279(53):55924.
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The synaptic protein UNC-18 is phosphorylated by protein kinase C.
Sassa T, Ogawa H, Kimoto M, Hosono R.
Neurochemistry International 1996 Nov; 29(5):543.
Application:WB-Re, N/A, Recombinant protein.
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Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.
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