Creb1 (phospho S133) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k Phospho-Creb1 S133 (Left Lane). The right lane is indicative that the specific immunolabeling of the Creb1 protein is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Creb1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S133 of rat Creb1.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Sheep, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of a forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45k Phospho-Creb1 S133 (Left Lane). The right lane is indicative that the specific immunolabeling of the Creb1 protein is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min). -
Gene Info — Creb1
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Publication Reference
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What turns CREB on?
Johannessen M, Delghandi MP, Moens U.
Cellular Signalling 2004 Nov; 16(11):1211.
Application：WB, Human, Human cancers, Mammalian cells.
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CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events.
Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia Z, Greenberg ME.
Neuron 2002 Apr; 34(2):221.
Application：WB-Tr, Monkey, Rat, Cortical neurons, COS cells.
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CREB: a stimulus-induced transcription factor activated by a diverse array of extracellular signals.
Shaywitz AJ, Greenberg ME.
Annual Review of Biochemistry 1999 Jan; 68:821.
Application：WB-Ce, WB-Tr, Human, Rat, Hippocampal neurons, Mammalian cells, PC-12 cells.
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What turns CREB on?
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