Grin2b (phospho Y1472) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y1472 of rat Grin2b.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
Specificity
Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. This antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either l-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y1472) polyclonal antibody (Cat # PAB9673). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Grin2b
-
Publication Reference
-
Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function.
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD.
The Journal of Biological Chemistry 2003 Jan; 278(13):11020.
Application:IP, WB, Rat, Rat hippocampus.
-
NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity.
Carroll RC, Zukin RS.
Trends in Neurosciences 2002 Nov; 25(11):571.
-
Trafficking of NMDA receptors.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.
Annual Review of Pharmacology and Toxicology 2002 Jan; 43:335.
-
Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com