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Last updated: 2016/12/4
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Grin2b (phospho Y1472) polyclonal antibody

  • Catalog # : PAB9673
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding Y1472 of rat Grin2b.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 180
  • Reactivity:
  • Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
  • Specificity:
  • Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. This antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either l-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • 3.
  • Trafficking of NMDA receptors.
    Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.Annu Rev Pharmacol Toxicol. 2003;43:335-58. Epub 2002 Jan 10.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y1472) polyclonal antibody (Cat # PAB9673). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • Grin2b
  • Gene Alias:
  • -
  • Gene Description:
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2B
  • Other Designations:
  • glutamate receptor, ionotropic, NMDA2B
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