Rabbit polyclonal antibody raised against synthetic phosphopeptide of Grin2b.
Synthetic phosphopeptide corresponding to residues surrounding Y1472 of rat Grin2b.
Theoretical MW (kDa):
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
Specific for the ~180k NMDAR NR2B-subunit protein phosphorylated at Tyr1472 in Western blots. This antibody also labels proteins of ~65k and ~115k. Immunolabeling is completely blocked by blocked by either l-Ptase or by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Trafficking of NMDA receptors. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.Annu Rev Pharmacol Toxicol. 2003;43:335-58. Epub 2002 Jan 10.
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho Y1472) polyclonal antibody (Cat # PAB9673). The immunolabeling is completely eliminated by treatment with lambda-Ptase.