Ppp1r1b (phospho T75) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k Ppp1r1b phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Ppp1r1b (phospho T75) polyclonal antibody (Cat # PAB9670). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Ppp1r1b.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T75 of rat Ppp1r1b.
Host
Rabbit
Theoretical MW (kDa)
32
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat caudate lysate showing specific immunolabeling of the ~32k Ppp1r1b phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Ppp1r1b (phospho T75) polyclonal antibody (Cat # PAB9670). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Ppp1r1b
Entrez GeneID
360616Protein Accession#
Q6J4I0Gene Name
Ppp1r1b
Gene Alias
Darpp-32, Darpp32
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 1B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
dopamine- and cAMP-regulated phosphoprotein DARPP-32|neuronal phosphoprotein DARPP-32
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Publication Reference
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Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine.
Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G.
Nature 2002 Aug; 418(6899):774.
Application：WB-Ti, Mouse , Mouse dorsal striatum.
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DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens.
Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Fienberg AA, Leslie SW, Gonzales RA, Morrisett RA.
Nature Neuroscience 2002 Jul; 5(7):641.
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Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac).
Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P.
PNAS 2002 Mar; 99(5):3182.
Application：WB-Ti, Mouse, Mouse brain.
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Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine.
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