Camk2a/Camk2b (phospho T286) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat brain lysate showing specific immunolabeling of the ~50 KDa- and the ~60 KDa Camk2a/Camk2b phosphorylated at Thr286 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Camk2a/Camk2b T286. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Camk2a/Camk2b.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T286 of rat Camk2a/Camk2b.
Host
Rabbit
Theoretical MW (kDa)
50, 60
Reactivity
Clawed frog, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 1 mg/mL BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brain lysate showing specific immunolabeling of the ~50 KDa- and the ~60 KDa Camk2a/Camk2b phosphorylated at Thr286 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-Camk2a/Camk2b T286. The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Camk2b
Entrez GeneID
24245Protein Accession#
P11275Gene Name
Camk2b
Gene Alias
Ck2b
Gene Description
calcium/calmodulin-dependent protein kinase (CaM kinase) II beta
Gene Ontology
HyperlinkGene Summary
beta subunit|Ca2+/calmodulin-dependent protein kinase II beta|calcium/calmodulin-dependent protein kinase II beta subunit|calcium/calmodulin-dependent pr
Other Designations
Ca++/calmodulin-dependent protein kinase II beta subunit|Ca++/calmodulin-dependent protein kinase II, beta subunit|Ca2+/calmodulin-dependent protein kinase II beta|calcium/calmodulin-dependent protein kinase II beta subunit|calcium/calmodulin-dependent pr
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Gene Info — Camk2a
Entrez GeneID
25400Protein Accession#
P11275Gene Name
Camk2a
Gene Alias
PK2CDD, PKCCD
Gene Description
calcium/calmodulin-dependent protein kinase II alpha
Gene Ontology
HyperlinkOther Designations
Ca2+/calmodulin-dependent protein kinase II alpha|alpha CaM kinase II|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha subunit
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Publication Reference
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Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats.
Meng F, Guo J, Zhang Q, Song B, Zhang G.
Brain Research 2003 Mar; 967(1-2):161.
Application：WB-Ti, Rat, Hippocampal.
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Translocation of CaM kinase II to synaptic sites in vivo.
Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR.
Nature Neuroscience 2003 Mar; 6(3):217.
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Inhibitory autophosphorylation of CaMKII controls PSD association, plasticity, and learning.
Elgersma Y, Fedorov NB, Ikonen S, Choi ES, Elgersma M, Carvalho OM, Giese KP, Silva AJ.
Neuron 2002 Oct; 36(3):493.
Application：WB-Ti, Mouse, Hippocampal.
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Autophosphorylated calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) reversibly targets to and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in cerebral ischemia and reperfusion in hippocampus of rats.
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