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Last updated: 2016/12/4
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Syt1 (phospho T202) polyclonal antibody

  • Catalog # : PAB9667
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of synaptotagmin.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T202 of rat synaptotagmin.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 60, 62
  • Reactivity:
  • Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k Syt1 phosphorylated at Thr202 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Syt1 (phospho T202) polyclonal antibody (Cat # PAB9667). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
  • Immunofluorescence
  • Immunofluorescence
  • Syt1 (phospho T202) polyclonal antibody (Cat # PAB9667) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label immunostaining of 14 DIV cortical neurons.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
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