ERK/MAPK (phospho T202/Y204) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of human T-47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERK/MAPK.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of rat ERK/MAPK.
Host
Rabbit
Theoretical MW (kDa)
42, 44
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of human T-47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Publication Reference
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Molecular recognitions in the MAP kinase cascades.
Tanoue T, Nishida E.
Cellular Signalling 2003 May; 15(5):455.
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Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.
Johnson GL, Lapadat R.
Science 2002 Dec; 298(5600):1911.
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Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD.
Annual Review of Pharmacology and Toxicology 2002 Apr; 42:135.
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Molecular recognitions in the MAP kinase cascades.
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