Mapk14 (phospho T180/Y182) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 Mapk14 protein phosphorylated at Thr180 and Tyr182.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mapk14.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T180/Y182 of rat Mapk14.
Host
Rabbit
Theoretical MW (kDa)
39
Reactivity
Bovine, Chicken, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:250-500)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 Mapk14 protein phosphorylated at Thr180 and Tyr182.Immunohistochemistry
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Gene Info — Mapk14
Entrez GeneID
81649Protein Accession#
P70618Gene Name
Mapk14
Gene Alias
CSBP, CSPB1, Csbp1, Csbp2, Exip, Hog, MGC105413, Mxi2, Prkm14, Prkm15, RK, Sapk2A, p38, p38Hog, p38alpha
Gene Description
mitogen activated protein kinase 14
Gene Ontology
HyperlinkOther Designations
Csaids binding protein|MAP kinase 2|MAP kinase Mxi2|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|cytokine-supressive anti-inflammatory drug binding protein|mitogen-activated protein kinase 14|p38 MAP kinase|p38 mit
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Publication Reference
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Vascular barrier protective effects of pellitorine in LPS-induced inflammation in vitro and in vivo.
Lee W, Ku SK, Min BW, Lee S, Jee JG, Kim JA, Bae JS.
Fitoterapia 2014 Jan; 92:177.
Application：WB-Ce, Human, HUVECs.
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Barrier protective effects of piperlonguminine in LPS-induced inflammation in vitro and in vivo.
Lee W, Yoo H, Kim JA, Lee S, Jee JG, Lee MY, Lee YM, Bae JS.
Food and Chemical Toxicology 2013 Aug; 58:149.
Application：WB, Human, HUVECs.
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Differential role of PTK, ERK and p38 MAPK in superoxide impairment of NMDA cerebrovasodilation.
Philip S, Armstead WM.
Brain Research 2003 Jul; 979(1-2):98.
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Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.
Johnson GL, Lapadat R.
Science 2002 Dec; 298(5600):1911.
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In the cellular garden of forking paths: how p38 MAPKs signal for downstream assistance.
Shi Y, Gaestel M.
Biological Chemistry 2002 Oct; 383(10):1519.
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Vascular barrier protective effects of pellitorine in LPS-induced inflammation in vitro and in vivo.
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