Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gsk3b.
Synthetic phosphopeptide corresponding to residues surrounding S9 of rat Gsk3b.
Theoretical MW (kDa):
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Also weakly labels the ~51k Gsk3b band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Western blot of rat cortex lysate showing specific immunolabeling of the ~46k Gsk3b protein phosphorylated at Ser9 (Control, lane 1). Immunolabeling is blocked by the phospho-peptide (Peptide, lane 2) used as antigen but not by the corresponding dephosphopeptide (not shown).