Gsk3b (phospho S9) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~46k Gsk3b protein phosphorylated at Ser9 (Control, lane 1). Immunolabeling is blocked by the phospho-peptide (Peptide, lane 2) used as antigen but not by the corresponding dephosphopeptide (not shown).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gsk3b.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S9 of rat Gsk3b.
Host
Rabbit
Theoretical MW (kDa)
46
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Specificity
Also weakly labels the ~51k Gsk3b band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~46k Gsk3b protein phosphorylated at Ser9 (Control, lane 1). Immunolabeling is blocked by the phospho-peptide (Peptide, lane 2) used as antigen but not by the corresponding dephosphopeptide (not shown). -
Gene Info — Gsk3b
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Publication Reference
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WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
Papkoff J, Aikawa M.
Biochemical and Biophysical Research Communications 1998 Jun; 247(3):851.
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Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling.
Sutherland C, Leighton IA, Cohen P.
The Biochemical Journal 1993 Nov; 296 ( Pt 1):15.
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WNT-1 and HGF regulate GSK3 beta activity and beta-catenin signaling in mammary epithelial cells.
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