Gria1 (phospho S831) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k Gria1 protein phosphorylated at Ser831 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gria1 (phospho S831) polyclonal antibody (Cat # PAB9661). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gria1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S831 of rat Gria1.
Host
Rabbit
Theoretical MW (kDa)
100
Reactivity
Chicken, Dog, Human, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100k Gria1 protein phosphorylated at Ser831 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gria1 (phospho S831) polyclonal antibody (Cat # PAB9661). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Gria1
Entrez GeneID
50592Protein Accession#
P19490Gene Name
Gria1
Gene Alias
gluR-A
Gene Description
glutamate receptor, ionotropic, AMPA 1
Gene Ontology
HyperlinkGene Summary
ionotropic
Other Designations
glutamate receptor A|glutamate receptor subunit GluR1|glutamate receptor, ionotropic, AMPA1 (alpha 1)
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Publication Reference
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Postsynaptic protein phosphorylation and LTP.
Soderling TR, Derkach VA.
Trends in Neurosciences 2000 Feb; 23(2):75.
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Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II.
Mammen AL, Kameyama K, Roche KW, Huganir RL.
The Journal of Biological Chemistry 1997 Dec; 272(51):32528.
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Characterization of multiple phosphorylation sites on the AMPA receptor GluR1 subunit.
Roche KW, O'Brien RJ, Mammen AL, Bernhardt J, Huganir RL.
Neuron 1996 Jun; 16(6):1179.
Application：WB-Tr, Human, HEK 293 cells.
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Postsynaptic protein phosphorylation and LTP.
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