Syn1 (phospho S62/S67) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific labeling of the ~78k Syn1 protein phosphorylated at Ser 62,67 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Syn1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S62/S67 of rat Syn1.
Host
Rabbit
Theoretical MW (kDa)
78
Reactivity
Bovine, Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific labeling of the ~78k Syn1 protein phosphorylated at Ser 62,67 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown). -
Gene Info — Syn1
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Publication Reference
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Regulation of neurotransmitter release by synapsin III.
Feng J, Chi P, Blanpied TA, Xu Y, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P.
Journal of Neuroscience 2002 Jun; 22(11):4372.
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A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P.
Nature Neuroscience 2002 Apr; 5(5):431.
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Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals.
Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC Jr, Greengard P, Czernik AJ.
The Journal of Neuroscience 2001 Oct; 21(20):7944.
Application：WB, Rat, Synaptosomes generated from cerebral cortices.
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Regulation of neurotransmitter release by synapsin III.
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