Syn1 (phospho S603) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~78k Syn1 phosphorylated at Ser603 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Syn1 (phospho S603) polyclonal antibody (Cat # PAB9658). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Syn1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S603 of rat Syn1.
Host
Rabbit
Theoretical MW (kDa)
78
Reactivity
Bovine, Clawed frog, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~78k Syn1 phosphorylated at Ser603 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Syn1 (phospho S603) polyclonal antibody (Cat # PAB9658). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Syn1
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Publication Reference
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Synapsin I is phosphorylated at Ser603 by p21-activated kinases (PAKs) in vitro and in PC12 cells stimulated with bradykinin.
Sakurada K, Kato H, Nagumo H, Hiraoka H, Furuya K, Ikuhara T, Yamakita Y, Fukunaga K, Miyamoto E, Matsumura F, Matsuo YI, Naito Y, Sasaki Y.
The Journal of Biological Chemistry 2002 Nov; 277(47):45473.
Application：ELISA, IF, WB-Ce, Human, PC12 cells, Supernatant from PC12 cells lysate.
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Regulation of neurotransmitter release by synapsin III.
Feng J, Chi P, Blanpied TA, Xu Y, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P.
Journal of Neuroscience 2002 Jun; 22(11):4372.
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A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth.
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P.
Nature Neuroscience 2002 Apr; 5(5):431.
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Synapsin I is phosphorylated at Ser603 by p21-activated kinases (PAKs) in vitro and in PC12 cells stimulated with bradykinin.
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