Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control, lane 1). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep, lane 2) but not by the corresponding dephosphopeptide (not shown).
Immunostaining of mouse hippocampal cryosections with 14-3-3 (phospho S58) polyclonal antibody (Cat # PAB9657) (green). GFAP positive astrocytes (red) and DAPI labeled nuclei (blue). Photo courtesty of Robert Wine.