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Last updated: 2016/12/4
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14-3-3 (phospho S58) polyclonal antibody

  • Catalog # : PAB9657
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of 14-3-3 protein.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S58 of rat 14-3-3 protein.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 29
  • Reactivity:
  • Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Sheep, Zebra fish
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot of rat brainstem lysate showing specific immuno- labeling of the ~29k 14-3-3 protein phosphorylated at Ser58 (Control, lane 1). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep, lane 2) but not by the corresponding dephosphopeptide (not shown).
  • Immunofluorescence
  • Immunofluorescence
  • Immunostaining of mouse hippocampal cryosections with 14-3-3 (phospho S58) polyclonal antibody (Cat # PAB9657) (green). GFAP positive astrocytes (red) and DAPI labeled nuclei (blue). Photo courtesty of Robert Wine.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
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