Mapt (phospho S416) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Mapt.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S416 of rat Mapt.
Host
Rabbit
Theoretical MW (kDa)
59, 65, 68
Reactivity
Bovine, Dog, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown). -
Gene Info — Mapt
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Publication Reference
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Disruption of the cytoskeleton in Alzheimer's disease.
Lee VM.
Current Opinion in Neurobiology 1995 Oct; 5(5):663.
Application：IHC, WB-Ti, Human, Rat, Brains.
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Expression of separate isoforms of human tau protein: correlation with the tau pattern in brain and effects on tubulin polymerization.
Goedert M, Jakes R.
The EMBO Journal 1990 Dec; 9(13):4225.
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Phosphorylation of microtubule-associated protein tau: identification of the site for Ca2(+)-calmodulin dependent kinase and relationship with tau phosphorylation in Alzheimer tangles.
Steiner B, Mandelkow EM, Biernat J, Gustke N, Meyer HE, Schmidt B, Mieskes G, Soling HD, Drechsel D, Kirschner MW, et al..
The EMBO Journal 1990 Nov; 9(11):3539.
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Disruption of the cytoskeleton in Alzheimer's disease.
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