Gap43 (phospho S41) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gap43 (phospho S41) polyclonal antibody (Cat # PAB9654). The immunolabeling of Gap43 is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Gap43.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S41 of rat Gap43.
Host
Rabbit
Theoretical MW (kDa)
50
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Specificity
Specific for the ~50k Gap43 protein phosphorylated at Ser41. In some tissues This antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap43 antibody, that may be a Gap43 aggregate or oligomer.
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Gap43 (phospho S41) polyclonal antibody (Cat # PAB9654). The immunolabeling of Gap43 is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Gap43
Entrez GeneID
29423Protein Accession#
P07936Gene Name
Gap43
Gene Alias
Basp2, MGC156666
Gene Description
growth associated protein 43
Gene Ontology
HyperlinkGene Summary
membrane attached signal protein 2|growth accentuating protein 43
Other Designations
brain abundant, membrane attached signal protein 2|growth accentuating protein 43
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Publication Reference
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A point mutant of GAP-43 induces enhanced short-term and long-term hippocampal plasticity.
Hulo S, Alberi S, Laux T, Muller D, Caroni P.
The European Journal of Neuroscience 2002 Jun; 15(12):1976.
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Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site.
He Q, Dent EW, Meiri KF.
Journal of Neuroscience 1997 May; 17(10):3515.
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GAP-43: an intrinsic determinant of neuronal development and plasticity.
Benowitz LI, Routtenberg A.
Trends in Neurosciences 1997 Feb; 20(2):84.
Application：IHC, WB-Ti, Human, Human brains.
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A point mutant of GAP-43 induces enhanced short-term and long-term hippocampal plasticity.
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