Western blot of recombinant phospho- and dephospho-Th showing selective immunolabeling by the phospho-specific antibody of the ~60k Th phosphorylated at Ser40. The pan-specific antibody (anti-pan-Th) recognized both the phospho- and dephospho-Th; while most importantly, the Th (phospho S40) polyclonal antibody (Cat # PAB9653) recognized only phospho-Th.
Immunohistochemistry (Frozen sections)
Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and Th (phospho S40) polyclonal antibody (Cat # PAB9653). The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.