Th (phospho S40) polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot of recombinant phospho- and dephospho-Th showing selective immunolabeling by the phospho-specific antibody of the ~60k Th phosphorylated at Ser40. The pan-specific antibody (anti-pan-Th) recognized both the phospho- and dephospho-Th; while most importantly, the Th (phospho S40) polyclonal antibody (Cat # PAB9653) recognized only phospho-Th.
Immunofluorescence
Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and Th (phospho S40) polyclonal antibody (Cat # PAB9653). The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Th.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S40 of rat Th.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (Frozen sections) (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot of recombinant phospho- and dephospho-Th showing selective immunolabeling by the phospho-specific antibody of the ~60k Th phosphorylated at Ser40. The pan-specific antibody (anti-pan-Th) recognized both the phospho- and dephospho-Th; while most importantly, the Th (phospho S40) polyclonal antibody (Cat # PAB9653) recognized only phospho-Th.Immunohistochemistry (Frozen sections)
Immunofluorescence
Immunohistochemical staining of retina with the pan-tyrosine hydroxylase (pan-TH) and Th (phospho S40) polyclonal antibody (Cat # PAB9653). The pan-TH antibody shows extensive labeling in this photomicrograph of the retina. In contrast, the phospho-TH antibody selectively labels only the two amacrine cells in this light-stimulated retina example. -
Gene Info — Th
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Publication Reference
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Tyrosine hydroxylase phosphorylation: regulation and consequences.
Dunkley PR, Bobrovskaya L, Graham ME, von Nagy-Felsobuki EI, Dickson PW.
Journal of Neurochemistry 2004 Dec; 91(5):1025.
Application：WB-Ce, Bovine, Bovine adrenal chromaffin cells.
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Acute administration of cocaine regulates the phosphorylation of serine-19, -31 and -40 in tyrosine hydroxylase.
Jedynak JP, Ali SF, Haycock JW, Hope BT.
Journal of Neurochemistry 2002 Jul; 82(2):382.
Application：WB-Ti, Rat, Rat caudate samples.
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Striatal dopaminergic and serotonergic markers in human heroin users.
Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW.
Neuropsychopharmacology: Official Publication of the American College of Neuropsychopharmacology 2001 May; 24(5):561.
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Tyrosine hydroxylase phosphorylation: regulation and consequences.
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