Map2k5 (phospho S311/T315) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat testis lysate showing specific labeling of the ~49k Map2k5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Map2k5.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S311/T315 of rat Map2k5.
Host
Rabbit
Theoretical MW (kDa)
49
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat testis lysate showing specific labeling of the ~49k Map2k5 protein (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown). -
Gene Info — Map2k5
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Publication Reference
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Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.
Cameron SJ, Abe J, Malik S, Che W, Yang J.
The Journal of Biological Chemistry 2003 Oct; 279(2):1506.
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MEK5 overexpression is associated with metastatic prostate cancer, and stimulates proliferation, MMP-9 expression and invasion.
Mehta PB, Jenkins BL, McCarthy L, Thilak L, Robson CN, Neal DE, Leung HY.
Oncogene 2003 Mar; 22(9):1381.
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MEKK3 directly regulates MEK5 activity as part of the big mitogen-activated protein kinase 1 (BMK1) signaling pathway.
Chao TH, Hayashi M, Tapping RI, Kato Y, Lee JD.
The Journal of Biological Chemistry 1999 Dec; 274(51):36035.
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Differential role of MEK5alpha and MEK5beta in BMK1/ERK5 activation.
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