Rph3a (phospho S234) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234. Using Rph3a (phospho S234) polyclonal antibody (Cat # PAB9645).
Immunofluorescence
Rph3a (phospho S234) polyclonal antibody (Cat # PAB9645) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining of 14 DIV Cortical Neurons.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Rph3a.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S234 of rat Rph3a.
Host
Rabbit
Theoretical MW (kDa)
82
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234. Using Rph3a (phospho S234) polyclonal antibody (Cat # PAB9645).Immunofluorescence
Rph3a (phospho S234) polyclonal antibody (Cat # PAB9645) and PSD-95 polyclonal antibody (Cat # PAB9623) double-label Immunostaining of 14 DIV Cortical Neurons. -
Gene Info — Rph3a
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Publication Reference
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MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.
Brozzi F, Lajus S, Diraison F, Rajatileka S, Hayward K, Regazzi R, Molnár E, Váradi A.
Molecular Biology of the Cell 2012 Nov; 23(22):4444.
Application：WB, Human, MIN6 beta cells.
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Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction.
Smith R, Petersén A, Bates GP, Brundin P, Li JY.
Neurobiology of Disease 2005 Dec; 20(3):673.
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Developmental regulation and specific brain distribution of phosphorabphilin.
Foletti DL, Scheller RH.
Journal of Neuroscience 2001 Aug; 21(15):5461.
Application：IF, IHC, WB-Ti, Rat, Rat brains, Rat cerebellar cortex.
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Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro.
Fykse EM, Li C, Südhof TC.
Journal of Neuroscience 1995 Mar; 15(3 Pt 2):2385.
Application：IP, WB-Re, WB-Ti, WB-Tr, Monkey, Rat, COS-7 cells, Rat brain proteins.
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MyRIP interaction with MyoVa on secretory granules is controlled by the cAMP-PKA pathway.
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