Tph2 (phospho S19) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Tph2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S19 of rat Tph2.
Host
Rabbit
Theoretical MW (kDa)
55
Reactivity
Bovine, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Recombinant protein)
Western blot of recombinant tryptophan hydroxylase incubated in the absence (Control, lane 1) and presence of Ca2+/calmodulin dependent kinase II (CaMKII, lane 2) showing specific immunolabeling of the ~55k Tph2 protein phosphorylated at Ser19. -
Gene Info — Tph2
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Publication Reference
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Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II.
Kuhn DM, Sakowski SA, Geddes TJ, Wilkerson C, Haycock JW.
Journal of Neurochemistry 2007 Nov; 103(4):1567.
Application:WB-Re, Recombinant protein.
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Different properties of the central and peripheral forms of human tryptophan hydroxylase.
McKinney J, Knappskog PM, Haavik J.
Journal of Neurochemistry 2005 Jan; 92(2):311.
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A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis.
Martinez A, Knappskog PM, Haavik J.
Current Medicinal Chemistry 2001 Jul; 8(9):1077.
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Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II.
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