Marcks (phospho S152/S156) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Marcks.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S152/S156 of rat Marcks.
Host
Rabbit
Theoretical MW (kDa)
87
Reactivity
Bovine, Chicken, Clawed frog, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brain lysate showing specific immunolabeling of the ~87k Marcks protein phosphorylated at Serr152,156 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase, lane 2). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Marcks Ser152,156 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — Marcks
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Publication Reference
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PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163.
Palmer RH, Schonwasser DC, Rahman D, Pappin DJ, Herget T, Parker PJ.
FEBS Letters 1996 Jan; 378(3):281.
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Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein.
Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ.
The Journal of Biological Chemistry 1994 Mar; 269(12):9361.
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Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain.
Ouimet CC, Wang JK, Walaas SI, Albert KA, Greengard P.
Journal of Neuroscience 1990 May; 10(5):1683.
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PRK1 phosphorylates MARCKS at the PKC sites: serine 152, serine 156 and serine 163.
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