Trafficking of NMDA receptors. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS.Annu Rev Pharmacol Toxicol. 2003;43:335-58. Epub 2002 Jan 10.
Western Blot (Tissue lysate)
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k Grin2b subunit of the NMDAR phosphorylated at Ser1480 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Grin2b (phospho S1480) polyclonal antibody (Cat # PAB9641). The immunolabeling is completely eliminated by treatment with lambda-Ptase.