Syt1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Syt1.
Immunogen
A synthetic peptide corresponding to rat Syt1.
Host
Rabbit
Theoretical MW (kDa)
65
Reactivity
Bovine, Chicken, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific labeling of the ~65k Syt1 protein. Using Syt1 polyclonal antibody (Cat # PAB9639). -
Gene Info — Syt1
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Publication Reference
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Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size.
Poskanzer KE, Fetter RD, Davis GW.
Neuron 2006 Apr; 50(1):49.
Application:IF, Flies, Synapses.
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Ca2+-dependent synaptotagmin binding to SNAP-25 is essential for Ca2+-triggered exocytosis.
Zhang X, Kim-Miller MJ, Fukuda M, Kowalchyk JA, Martin TF.
Neuron 2002 May; 34(4):599.
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Synaptotagmin controls and modulates synaptic-vesicle fusion in a Ca(2+)-dependent manner.
Littleton JT, Bellen HJ.
Trends in Neurosciences 1995 Apr; 18(4):177.
Application:WB-Ce, WB-Tr, Mouse, Nematoda, Neuronal cells.
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Discrete residues in the c(2)b domain of synaptotagmin I independently specify endocytic rate and synaptic vesicle size.
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