Gja1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k Gja1 protein. Using Gja1 polyclonal antibody (Cat # PAB9631).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Gja1.
Immunogen
A synthetic peptide corresponding to C-terminus rat Gja1.
Host
Rabbit
Theoretical MW (kDa)
43
Reactivity
Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Sheep, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cerebellar lysate showing specific immunolabeling of the ~43k Gja1 protein. Using Gja1 polyclonal antibody (Cat # PAB9631). -
Gene Info — Gja1
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Publication Reference
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Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
Bao X, Reuss L, Altenberg GA.
The Journal of Biological Chemistry 2004 May; 279(19):20058.
Application：WB, Recombinant protein.
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Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
Bao X, Altenberg GA, Reuss L.
American Journal of Physiology. Cell Physiology 2004 Mar; 286(3):C647.
Application：IP, WB, Frog, Frog oocytes.
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Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells.
Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T.
FEBS Letters 2002 Nov; 531(2):132.
Application：IF, WB-Tr, Human, Rat, HeLa, Rat primary granulosa cells.
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Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368.
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