ERK/MAPK polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ERK/MAPK.
Immunogen
A synthetic peptide corresponding to C-terminus rat ERK/MAPK.
Host
Rabbit
Theoretical MW (kDa)
42, 44
Reactivity
Mouse, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat hippocampal homogenate showing specific immunolabeling of ~42-44k ERK/MAPK protein. Using ERK/MAPK polyclonal antibody (Cat # PAB9630). -
Publication Reference
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Molecular recognitions in the MAP kinase cascades.
Tanoue T, Nishida E.
Cellular Signalling 2003 May; 15(5):455.
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Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.
Johnson GL, Lapadat R.
Science 2002 Dec; 298(5600):1911.
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Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD.
Annual Review of Pharmacology and Toxicology 2002 Apr; 42:135.
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Molecular recognitions in the MAP kinase cascades.
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