Ppp1r1b polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k Ppp1r1b protein. Using Ppp1r1b polyclonal antibody (Cat # PAB9626).
Immunofluorescence
Immunochemical staining of cultured mouse caudate neurons with Ppp1r1b polyclonal antibody (Cat # PAB9626). Cells and photo courtesy of QBM Cell Science.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Ppp1r1b.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus rat Ppp1r1b.
Host
Rabbit
Theoretical MW (kDa)
32
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of a rat hippocampal lysate showing specific immunolabeling of the ~32k Ppp1r1b protein. Using Ppp1r1b polyclonal antibody (Cat # PAB9626).Immunofluorescence
Immunochemical staining of cultured mouse caudate neurons with Ppp1r1b polyclonal antibody (Cat # PAB9626). Cells and photo courtesy of QBM Cell Science. -
Gene Info — Ppp1r1b
Entrez GeneID
360616Protein Accession#
Q6J4I0Gene Name
Ppp1r1b
Gene Alias
Darpp-32, Darpp32
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 1B
Gene Ontology
HyperlinkGene Summary
O
Other Designations
dopamine- and cAMP-regulated phosphoprotein DARPP-32|neuronal phosphoprotein DARPP-32
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Publication Reference
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Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine.
Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G.
Nature 2002 Aug; 418(6899):774.
Application：WB-Ti, Mouse , Mouse dorsal striatum.
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DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens.
Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Fienberg AA, Leslie SW, Gonzales RA, Morrisett RA.
Nature Neuroscience 2002 Jul; 5(7):641.
Application：WB-Ti, Rat, Rat brain.
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Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac).
Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P.
PNAS 2002 Mar; 99(5):3182.
Application：WB-Ti, Mouse, Mouse brain.
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Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine.
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