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Last updated: 2017/3/26
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Polo-Like Kinase (phospho T210) polyclonal antibody

  • Catalog # : PAB9614
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of Polo-Like Kinase.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T210 of human and rat Polo-Like Kinase.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 66
  • Reactivity:
  • Bovine, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k Polo-Like Kinase protein phosphorylated at Thr210 (control). The phosphospecificity of this abeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Polo-Like Kinase (phospho T210) polyclonal antibody (Cat # PAB9614). The mmunolabeling is completely eliminated by treatment with lambda-Ptase.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
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