Polo-Like Kinase (phospho T210) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k Polo-Like Kinase protein phosphorylated at Thr210 (control). The phosphospecificity of this abeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Polo-Like Kinase (phospho T210) polyclonal antibody (Cat # PAB9614). The mmunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Polo-Like Kinase.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T210 of human and rat Polo-Like Kinase.
Host
Rabbit
Theoretical MW (kDa)
66
Reactivity
Bovine, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k Polo-Like Kinase protein phosphorylated at Thr210 (control). The phosphospecificity of this abeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Polo-Like Kinase (phospho T210) polyclonal antibody (Cat # PAB9614). The mmunolabeling is completely eliminated by treatment with lambda-Ptase. -
Publication Reference
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Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1.
van de Weerdt BC, van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH.
Molecular and Cellular Biology 2005 Mar; 25(5):2031.
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Polo-like kinases (Plks) and cancer.
Takai N, Hamanaka R, Yoshimatsu J, Miyakawa I.
Oncogene 2005 Jan; 24(2):287.
Application：IHC, WB, Human, Human cancer.
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Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/Cdc20 activation and initiation of cytokinesis.
van Vugt MA, van de Weerdt BC, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RM, Medema RH.
The Journal of Biological Chemistry 2004 Aug; 279(35):36841.
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Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1.
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