Taok2 (phospho S181) polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~120k Taok2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Taok2 (phospho S181) polyclonal antibody (Cat # PAB9613). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Taok2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S181 of human, mouse and rat Taok2.
Host
Rabbit
Theoretical MW (kDa)
120
Reactivity
Clawed frog, Dog, Human, Mouse, Rat, Zebra fish
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat cortex lysate showing specific immunolabeling of the ~120k Taok2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Taok2 (phospho S181) polyclonal antibody (Cat # PAB9613). The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Publication Reference
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TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors.
Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH.
The Journal of Biological Chemistry 2003 Jun; 278(25):22278.
Application：IP, WB-Ce, Mouse , C2C12 cells.
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Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2.
Chen Z, Cobb MH.
The Journal of Biological Chemistry 2001 May; 276(19):16070.
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Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain.
Chen Z, Hutchison M, Cobb MH.
The Journal of Biological Chemistry 1999 Oct; 274(40):28803.
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TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors.
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