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Last updated: 2016/12/4
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MAP2K1 (phospho T386) polyclonal antibody

  • Catalog # : PAB9609
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T386 of human MAP2K1.
  • Host:
  • Rabbit
  • Theoretical MW (kDa):
  • 45
  • Reactivity:
  • Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot of human T-47D cells showing specific immunolabeling of the ~45k MAP2K1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the MAP2K1 (phospho T386) polyclonal antibody (Cat # PAB9609). The immunolabeling of MAP2K1 is completely eliminated by lambda Ptase.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5604
  • Gene Name:
  • MAP2K1
  • Gene Alias:
  • MAPKK1,MEK1,MKK1,PRKMK1
  • Gene Description:
  • mitogen-activated protein kinase kinase 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
  • Other Designations:
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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