MAP2K1 (phospho T386) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot of human T-47D cells showing specific immunolabeling of the ~45k MAP2K1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the MAP2K1 (phospho T386) polyclonal antibody (Cat # PAB9609). The immunolabeling of MAP2K1 is completely eliminated by lambda Ptase.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T386 of human MAP2K1.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot of human T-47D cells showing specific immunolabeling of the ~45k MAP2K1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: lambda Ptase) The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the MAP2K1 (phospho T386) polyclonal antibody (Cat # PAB9609). The immunolabeling of MAP2K1 is completely eliminated by lambda Ptase. -
Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
Q02750Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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Mechanisms of regulating the Raf kinase family.
Chong H, Vikis HG, Guan KL.
Cellular Signalling 2003 May; 15(5):463.
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Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD.
Annual Review of Pharmacology and Toxicology 2002 Apr; 42:135.
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Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.
Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG.
Journal of Biochemistry 1994 Aug; 116(2):304.
Application：WB-Tr, Mouse, CHO cells.
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Mechanisms of regulating the Raf kinase family.
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