IFNAR1 (phospho S535/S539) polyclonal antibody
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More Files
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Immunoprecipitation
Western blot of immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of IFNAR1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S535/S539 of human IFNAR1.
Host
Rabbit
Theoretical MW (kDa)
~110-130
Reactivity
Bovine, Dog, Human, Mouse, Primates, Rat, Sheep
Form
Liquid
Preparation Method
The antibody is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Purification
Affinity purification
Recommend Usage
Immunohistochemistry (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 100 ug/mL BSA).
Storage Instruction
Store at -20°C for short term storage is acceptable as aliquots may be taken without freeze/thaw due to the presence of 50% glycerol. For long term storage, store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
The molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 kDa - 130 kDa.
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Applications
Western Blot
Immunohistochemistry
Immunoprecipitation
Western blot of immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos). -
Gene Info — IFNAR1
Entrez GeneID
3454Protein Accession#
P17181Gene Name
IFNAR1
Gene Alias
AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC
Gene Description
interferon (alpha, beta and omega) receptor 1
Omim ID
107450Gene Ontology
HyperlinkOther Designations
alpha-type antiviral protein|beta-type antiviral protein|interferon-alpha receptor 1|interferon-alpha/beta receptor alpha chain|interferon-beta receptor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor.
Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY.
The EMBO Journal 2003 Oct; 22(20):5480.
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Cancer immunotherapy: the interferon-alpha experience.
Kirkwood J.
Seminars in Oncology 2002 Jun; 29(3 Suppl 7):18.
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Interferons alpha and beta as immune regulators--a new look.
Biron CA.
Immunity 2001 Jun; 14(6):661.
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SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor.
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