TP53 (phospho S392) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of TP53.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S392 of human TP53.
Host
Rabbit
Theoretical MW (kDa)
53
Reactivity
Human, Primates, Rat
Form
Liquid
Purification
Affinity purification
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k TP53 phosphorylated at Ser392 (Control).
The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase).
The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Phospho-TP53 S392 polyclonal antibody (Cat # PAB9604).
The immunolabeling is completely eliminated by treatment with lambda-Ptase. -
Gene Info — TP53
Entrez GeneID
7157Protein Accession#
P04637Gene Name
TP53
Gene Alias
FLJ92943, LFS1, TRP53, p53
Gene Description
tumor protein p53
Gene Ontology
HyperlinkGene Summary
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq
Other Designations
p53 antigen|p53 transformation suppressor|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53
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Interactome
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Pathway
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Disease
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Publication Reference
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Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability.
Kim YY, Park BJ, Kim DJ, Kim WH, Kim S, Oh KS, Lim JY, Kim J, Park C, Park SI.
FEBS Letters 2004 Aug; 572(1-3):92.
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Phosphorylation site interdependence of human p53 post-translational modifications in response to stress.
Saito S, Yamaguchi H, Higashimoto Y, Chao C, Xu Y, Fornace AJ Jr, Appella E, Anderson CW.
The Journal of Biological Chemistry 2003 Sep; 278(39):37536.
Application:WB, Human, A-549, HCT-116 cells.
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p53: good cop/bad cop.
Sharpless NE, DePinho RA.
Cell 2002 Jul; 110(1):9.
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Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability.
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