Products

Product Description

Goat anti-rabbit IgG recognizes rabbit IgG whole molecule. This secondary antibody was purified using a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogen

Rabbit IgG whole molecule.

Host

Goat

Reactivity

Rabbit

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, rabbit IgG and rabbit serum.

Form

Lyophilized

Conjugation

Unconjugated

Purification

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Conjugation Note

Recommend Usage

ELISA (1:50,000)
Immunohistochemistry (1:1,000-1:5,000)
Western Blot (1:5,000-1:25,000)
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from 0.02 mM potassium phosphate, 0.15 M NaCl, pH 7.2 (0.01% (w/v) sodium azide).

Storage Instruction

Store at 4°C on dry atmosphere prior to restoration.
After reconstitution with 5 mL of deionized water, store at -20°C or lower. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Aliquot to avoid repeated freezing and thawing.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.