Products

Product Description

Goat polyclonal antibody raised against mouse IgG F(c) fragment. This secondary antibody generated in goat detects specifically mouse IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Immunogen

Mouse IgG F(c) fragment.

Host

Goat

Reactivity

Mouse

Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, mouse IgG, mouse IgG F(c) and mouse serum. No reaction was observed against mouse IgG F(ab).

Form

Lyophilized

Conjugation

Unconjugated

Purification

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Conjugation Note

Recommend Usage

ELISA (1:20000-1:100000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.

Storage Buffer

Lyophilized from 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 (0.01% (w/v) sodium azide).

Storage Instruction

Store vial at 4°C prior to restoration.
After reconstitution with 5.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Note

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.