Goat anti-mouse IgG recognizes mouse IgG F(c) fragment. This secondary antibody was purified using antigen affinity chromatography.
Mouse IgG F(c) fragment
No reaction was observed against anti-Pepsin, anti-goat IgG F(c), mouse IgG F(ab')2 or bovine, horse and human serum proteins.
This product was prepared from monospecific antiserum by immunoaffinity chromatography, followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
ELISA (1:10000) WB (1:2000-1:10000) IHC (1:1000-1:5000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.