Goat Anti-Human IgG F(c) secondary antibody
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Specification
Product Description
Goat polyclonal antibody raised against human IgG F(c) fragment.
Immunogen
Human IgG F(c) fragment.
Host
Goat
Reactivity
Human
Specificity
IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG F(c) and human serum. No reaction was observed against human IgG F(ab).
Form
Lyophilized
Conjugation
Unconjugated
Purity
This product was prepared from monospecific antiserum by a delipidation and defibrination.
Format
Antiserum
Recommend Usage
ELISA (1:20000-1:100000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.01 M potassium phosphate, 0.15 M sodium chloride, pH 7.2 (0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 2.0 mL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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