Goat Anti-Dog IgG F(c) secondary antibody
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Specification
Product Description
Goat anti-dog IgG recognizes dog IgG F(c) fragment. This secondary antibody was purified using antigen affinity chromatography.
Immunogen
Dog IgG F(c) fragment
Host
Goat
Reactivity
Dog
Specificity
IgG F(c). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, dog IgG, dog IgG F(c) and dog serum. No reaction was observed against dog IgG F(ab).
Form
Liquid
Conjugation
Unconjugated
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using dog IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Recommend Usage
ELISA (1:20,000-1:100,000)
Immunohistochemistry (1:1,000-1:5,000)
Western Blot (1:2,000-1:10,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C prior to opening. For long term storage, store at -20°C or below.
Aliquot to avoid repeated freezing and thawing.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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