Enpp2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of Enpp2 from zebrafish embryo lysate by Enpp2 polyclonal antibody ( Cat # PAB8520 ) (1:500, 4°C, overnight). Instead of a predicted 93.6 KDa band, we detected a ~50KDa single band from shield stage of wild type embryos (lane 1) on a 12% SDS-PAGE, and this band can be efficiently knockdown by Enpp2 morpholino oligonucleotides (land 2 and 3). This phenomenon is probably due to a cleavage of endogenous Enpp2 under reducing condition of SDS-PAGE as previously reported in human and mice. (Journal of Biochemistry, 283(12):7776-7789)
(By courtesy of Shih-Lei Lai1 and Shyh-Jye Lee1-4; 1Institute of Zoology, 2Department of Life Science, 3Center for Biotechnology, 4Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University)
Immunofluorescence
Immunofluorescence analysis of mouse spinal cord, using Enpp2 polyclonal antibody (Cat # PAB8520) .
Immunofluorescence
Immunofluorescence analysis of mouse thalamus, using Enpp2 polyclonal antibody (Cat # PAB8520) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Enpp2.
Immunogen
A synthetic peptide corresponding to mouse Enpp2.
Sequence
LKTYLHTYESEI
Host
Rabbit
Reactivity
Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (0.2-2.0 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of Enpp2 from zebrafish embryo lysate by Enpp2 polyclonal antibody ( Cat # PAB8520 ) (1:500, 4°C, overnight). Instead of a predicted 93.6 KDa band, we detected a ~50KDa single band from shield stage of wild type embryos (lane 1) on a 12% SDS-PAGE, and this band can be efficiently knockdown by Enpp2 morpholino oligonucleotides (land 2 and 3). This phenomenon is probably due to a cleavage of endogenous Enpp2 under reducing condition of SDS-PAGE as previously reported in human and mice. (Journal of Biochemistry, 283(12):7776-7789)
(By courtesy of Shih-Lei Lai1 and Shyh-Jye Lee1-4; 1Institute of Zoology, 2Department of Life Science, 3Center for Biotechnology, 4Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University)Immunofluorescence
Immunofluorescence analysis of mouse spinal cord, using Enpp2 polyclonal antibody (Cat # PAB8520) .Immunofluorescence
Immunofluorescence analysis of mouse thalamus, using Enpp2 polyclonal antibody (Cat # PAB8520) . -
Gene Info — Enpp2
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Publication Reference
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The N-terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide.
Koike S, Keino-Masu K, Ohto T, Masu M.
Genes to Cells 2006 Feb; 11(2):133.
Application：IF, WB-Tr, Human, Monkey, COS-7, HEK 293 cells.
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NPP-type ectophosphodiesterases: unity in diversity.
Stefan C, Jansen S, Bollen M.
Trends in Biochemical Sciences 2005 Oct; 30(10):542.
Application：WB, Human, Human mammalian cells.
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Identification of autotaxin as a neurite retraction-inducing factor of PC12 cells in cerebrospinal fluid and its possible sources.
Sato K, Malchinkhuu E, Muraki T, Ishikawa K, Hayashi K, Tosaka M, Mochiduki A, Inoue K, Tomura H, Mogi C, Nochi H, Tamoto K, Okajima F.
Journal of Neurochemistry 2005 Feb; 92(4):904.
Application：WB, Monkey, Rat, COS-7, Rat neural cells.
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Prostatic acid phosphatase degrades lysophosphatidic acid in seminal plasma.
Tanaka M, Kishi Y, Takanezawa Y, Kakehi Y, Aoki J, Arai H.
FEBS Letters 2004 Jul; 571(1-3):197.
Application：WB, Human, Human biological fluids.
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The N-terminal hydrophobic sequence of autotaxin (ENPP2) functions as a signal peptide.
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