Western blot analysis of Enpp2 from zebrafish embryo lysate by Enpp2 polyclonal antibody ( Cat # PAB8520 ) (1:500, 4°C, overnight). Instead of a predicted 93.6 KDa band, we detected a ~50KDa single band from shield stage of wild type embryos (lane 1) on a 12% SDS-PAGE, and this band can be efficiently knockdown by Enpp2 morpholino oligonucleotides (land 2 and 3). This phenomenon is probably due to a cleavage of endogenous Enpp2 under reducing condition of SDS-PAGE as previously reported in human and mice. (Journal of Biochemistry, 283(12):7776-7789) (By courtesy of Shih-Lei Lai1 and Shyh-Jye Lee1-4; 1Institute of Zoology, 2Department of Life Science, 3Center for Biotechnology, 4Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University)
Immunofluorescence analysis of mouse spinal cord, using Enpp2 polyclonal antibody (Cat # PAB8520) .
Immunofluorescence analysis of mouse thalamus, using Enpp2 polyclonal antibody (Cat # PAB8520) .