PHOT1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of phototropin 1.
Immunogen
A synthetic peptide corresponding to arabidopsis phototropin 1.
Host
Rabbit
Reactivity
Thale cress
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of extract from Arabidopsis leaves, using Phototropin 1 polyclonal antibody (Cat # PAB8518) . Lane 1: wild type and lane 2: delection mutant for PHOT1/PHOT2 .Western Blot (Recombinant protein)
Western blot analysis of GST-GFP fusion protein in E coli. , using Phototropin 1 polyclonal antibody (Cat # PAB8518) . Lane 1: GST-GFP-PHOT1 N terminal and lane 2: GST-GFP-PHOT2 N terminal. -
Gene Info — PHOT1
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Publication Reference
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Chloroplast movement.
Wada M, Kagawa T, Sato Y.
Annual Review of Plant Biology 2003 Jan; 54:455.
Application:IF, Plant, Plant.
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The phototropin family as photoreceptors for blue light-induced chloroplast relocation.
Kagawa T.
Journal of Plant Research 2003 Feb; 116(1):77.
Application:WB, Plant, Plant cells.
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Chloroplast avoidance movement reduces photodamage in plants.
Kasahara M, Kagawa T, Oikawa K, Suetsugu N, Miyao M, Wada M.
Nature 2002 Dec; 420(6917):829.
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Blue light-induced chloroplast relocation.
Kagawa T, Wada M.
Plant & Cell Physiology 2002 Apr; 43(4):367.
Application:IF, Plant, Plant cells.
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The phototropin family of photoreceptors.
Briggs WR, Beck CF, Cashmore AR, Christie JM, Hughes J, Jarillo JA, Kagawa T, Kanegae H, Liscum E, Nagatani A, Okada K, Salomon M, Rüdiger W, Sakai T, Takano M, Wada M, Watson JC.
The Plant Cell 2001 May; 13(5):993.
Application:WB, Plant, Plant cells.
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Chloroplast movement.
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