PHOT2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of phototropin 2.
Immunogen
A synthetic peptide corresponding to amino acids 12-22 at N-terminus of arabidopsis phototropin 2
Sequence
NDAESLSERRSC
Host
Rabbit
Reactivity
Thale cress
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.1% proclin, 2.0% Block Ace)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot analysis of extract from Arabidopsis leaves, using Phototropin 2 polyclonal antibody (Cat # PAB8516) . Lane 1: mutant type lacking phototropin 2, lane 2: hetero type and lane 3: wild type.Western Blot (Recombinant protein)
Western blot analysis of GST fusion protein in E coli. , using Phototropin 2 polyclonal antibody (Cat # PAB8516) . Lane 1: GST-phototropin 1 N-terminal (without IPTG induction) , lane 2: GST-phototropin 1 N-terminal (with IPTG induction) , lane 3: GST-phototropin 2 N-terminal (without IPTG induction) and GST-phototropin 2 N-terminal (with IPTG induction) . -
Gene Info — PHOT2
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Publication Reference
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Chloroplast movement.
Wada M, Kagawa T, Sato Y.
Annual Review of Plant Biology 2003 Jan; 54:455.
Application:IF, Plant, Plant.
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The phototropin family as photoreceptors for blue light-induced chloroplast relocation.
Kagawa T.
Journal of Plant Research 2003 Feb; 116(1):77.
Application:WB, Plant, Plant cells.
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Chloroplast avoidance movement reduces photodamage in plants.
Kasahara M, Kagawa T, Oikawa K, Suetsugu N, Miyao M, Wada M.
Nature 2002 Dec; 420(6917):829.
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Blue light-induced chloroplast relocation.
Kagawa T, Wada M.
Plant & Cell Physiology 2002 Apr; 43(4):367.
Application:IF, Plant, Plant cells.
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The phototropin family of photoreceptors.
Briggs WR, Beck CF, Cashmore AR, Christie JM, Hughes J, Jarillo JA, Kagawa T, Kanegae H, Liscum E, Nagatani A, Okada K, Salomon M, Rüdiger W, Sakai T, Takano M, Wada M, Watson JC.
The Plant Cell 2001 May; 13(5):993.
Application:WB, Plant, Plant cells.
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Chloroplast movement.
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